A simple, precise, accurate, economic and reproducible UV spectrophotometric method has been developed for simultaneous estimation of Montelukast Sodium (MONTE) and Olopatadine Hydrochloride (OLO) by employing first order derivative zero crossing method in 0.1 N NaOH. The wavelength 295.18 nm (zero cross point of Olopatadine Hydrochloride) was selected for quantification of Montelukast Sodium and wavelength 303.65 nm (zero cross point of Montelukast Sodium) was selected for quantification of Olopatadine Hydrochloride The linearity was established over the concentration range of 20-40 µg/ml and 10-20 µg/ml for Montelukast Sodium and Olopatadine Hydrochloride with correlation coefficient r2 0.998 of both the drugs. The mean % recoveries were found to be in the range of 99.82 %–100.52 % and 100.42%–101.21% for Montelukast Sodium and Olopatadine Hydrochloride, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Montelukast Sodium and Olopatadine Hydrochloride in synthetic mixture.
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